Improving oedema disease diagnosis in pigs by detecting the vt2e toxin gen in oral fluid by qPCR
Here we report on the use of oral fluid (OF) and real time PCR (qPCR) as alternative methods in terms of sampling, representivness and cost.
28 September 2019
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Image 1. Oral Fluid sampling procedure: pigs chewing cotton rope
Image 2. Real time PCR instrument
(A) Number and geographic origin of positive (green) and negative (blue) samples in 18 farms tested for the vy2e gene by qPCR.
(B) Percentage of vt2e positive and negative farms.
vt2e qPCR from 16 pens in a VTEC-infected fattening swine unit.
| References | ||||
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