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Immunoproteomic Analysis of Bacterial Proteins of <em>Actinobacillus pleuropneumoniae</em> Serotype 1

by 5m Editor
21 August 2011, at 12:00am

One or more of the 42 novel immunoreactive factors identified by researchers at Nanjing Agricultural University could become vaccine candidates.

Actinobacillus pleuropneumoniae (APP) is one of the most important swine pathogens worldwide, according to Wei Zhang and collaeagues at Nanjing Agricultural University in China in a paper published in Proteome Science. Identification and characterisation of novel antigenic APP vaccine candidates are underway. In this present study, the Nanjing researchers used an immunoproteomic approach to identify APP protein antigens that may elicit an immune response in serotype 1 naturally infected swine and serotype 1 virulent strain S259-immunised rabbits.

Proteins from total cell lysates of serotype 1 APP were separated by two-dimensional electrophoresis (2DE). Western blot analysis revealed 21 immunoreactive protein spots separated in the pH 4-7 range and four spots in the pH 7-11 range with the convalescent sera from swine.

The researchers found five immunoreactive protein spots that separated in the pH 4-7 range and 2 in the pH 7-11 range with hyperimmune sera from S259-immunised rabbits. The proteins included the known antigens ApxIIA, protective surface antigen D15, outer membrane proteins P5, subunit NqrA. The remaining antigens are being reported as immunoreactive proteins in APP for the first time, to the authors' knowledge.

Zhang and co-authors concluded that they identified a total of 42 immunoreactive proteins of the APP serotype 1 virulent strain S259, which represented 32 different proteins, including some novel immunoreactive factors which could be researched as vaccine candidates.

Reference

Zhang W., J. Shao, G. Liu, F. Tang, Y. Lu, Z. Zhai, Y. Wang, Z. Wu, H. Yao and C. Lu. 2011. Immunoproteomic analysis of bacterial proteins of Actinobacillus pleuropneumoniae serotype 1. Proteome Science, 9:32 doi:10.1186/1477-5956-9-32

Further Reading

- You can view the full report by clicking here.


Further Reading

- Find out more information on Actinobacillus pleuropneumonia (APP) by clicking here.


August 2011