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Detection of Swine Transmissible Gastroenteritis Coronavirus Using Loop-Mediated Isothermal Amplification

by 5m Editor
23 August 2010, at 12:00am

Researchers at Shanghai University, China, have developed an improved method for the detection of the virus causing transmissible gastroenteritis (TGE) in pigs. Their paper is published in Virology Journal.

A conserved nucleic acid fragment of the nucleocapsid gene of Swine Transmissible Gastroenteritis Coronavirus (TGEV) was chosen as the target, six special primers were designed successfully.

Loop-mediated isothermal amplification (LAMP) was developed to detect the TGEV by incubation at 60°C for one hour and the product specificity was confirmed by HphI digestion. Standard curves with high accuracy for TGEV quantification was constructed by adding 1xSYBR greenin the LAMP reaction.

The assay established in this study was found to detect only the TGEV and no cross-reaction with other viruses, demonstrating its high specificity.

By using serial sample dilutions as templates, the detection limit of LAMP was about 10 pg RNA, 10 times more sensitive than that of PCR and could be comparable to the nest-PCR.

Reference

Qin Chen, Jian Li, Xue-En Fang and Wei Xiong. 2010. Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification. Virology Journal, 7:206. doi:10.1186/1743-422X-7-206

Further Reading

- You can view the full report (as a provisional PDF) by clicking here.


Further Reading

- Find out more information on transmissible gastroenteritis by clicking here.


August 2010