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Comparison of Two Commercial Real-time Reverse Transcriptase PCR Assays for PRRS

31 July 2012, at 12:00am

Neither of the two assays tested detected every positive case of porcine reproductive and respiratory syndrome (PRRS), according to new research conducted at Iowa State University.

Specimens were tested for PRRS virus by real-time reverse transcriptase polymerase chain reaction (RT-PCR) with two commercial agent-specific assays, report Dr Karen M. Harmon and colleagues at Iowa State University in a paper published recently in Journal of Swine Health and Production.

Result discrepancy between the two tests was confirmed by conventional RT-PCR, sequencing, or both on six of 423 clinical cases. Neither assay detected every positive case.

Reference

Harmon K.M., S.A. Abate, A.J. Chriswell, W.A. Chittick and E.L. Strait. 2012. Comparison of two commercial real-time reverse transcriptase polymerase chain reaction assays for porcine reproductive and respiratory syndrome virus. J Swine Health Prod. 2012;20(4):184–188.

Further Reading

You can view the full report by clicking here.


Further Reading

Find out more information on porcine reproductive respiratory syndrome (PRRS) by clicking here.


July 2012